Detecção não letal de mtDNA do monogenético Gyrodactylus mutabilitas que registrou pela primeira vez no muco branquial de Cyprinus carpio, Iraque

Autores

DOI:

https://doi.org/10.5965/223811712332024554

Palavras-chave:

Gyrodactylus mutabilitas, carpa comum, amostragem não letal, preferência de site, Iraque.

Resumo

 As espécies monogenéticas do gênero Gyrodactylus são nocivas à carpa comum Cyprinus carpio. Neste estudo, amostras de peixes foram coletadas na fazenda de peixes al-Tarmiyahm, em Bagdá, Iraque. A ocorrência do monogenético Gyrodactylus mutabilitas é relatada no presente estudo pela primeira vez em brânquias da carpa comum. São fornecidas caracterização morfológica e medidas deste parasita. A distribuição deste monogenético sobre os arcos branquiais dos hospedeiros mostrou uma preferência específica pelo lado direito, havia preferências óbvias para os segundos arcos branquiais registrados por este monogenético. Normalmente, o diagnóstico preciso do monogenético baseia-se em métodos tradicionais pela sua morfologia e só pode ser alcançado por dissecação post-mortem do peixe. Para apoiar a conservação e gestão da carpa comum na piscicultura, é urgentemente necessário um método não letal para a detecção de agentes patogénicos. Neste estudo, o esfregaço branquial parece fornecer um método útil para amostras de DNA de Gyrodactylus mutabilitas. Os gestores das pescas podem considerar este método uma ferramenta útil para avaliar o estado da infecção, o que pode impedir a propagação de qualquer possível infecção

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Referências

ALI M & SHAABAN F. 1984. Some species of parasites of freshwater fish raised in ponds and in Tigris-Al-Tharthar canal region. Seventh Sci. Mosul: Paper presented at the Conf. Iraqi Vet. Med. Assoc.

BAGGE A & VALTONEN E. 1999. Development of monogenean communities on the gills of roach fry (Rutilus rutilus). Parasitology 118: 479-487.

BAKER JR et al. 1998. Advances in parasitology. London: Academic Press.

BAKKE T A et al. 2007. The biology of gyrodactylid monogeneans: the “Russian-doll killers”. Advances in parasitology 64: 161-460.

BARZEGAR M et al. 2018. Gyrodactylus (Monogenea, Gyrodactylidae) parasite fauna of fishes in some rivers of the southern Caspian Sea basin in Mazandaran province. Iran J Vet Med 12: 35-44.

BONDAD-REANTASO MG et al. 2005. Disease and health management in Asian aquaculture. Veterinary parasitology 132: 249-272.

BUCHMANN K. 2012. Gyrodactylus salaris and Gyrodactylus derjavinoides. In Fish parasites: pathobiology and protection. Wallingford: CABI. p. 193-208.

BUENO-SILVA M & BOEGER WA. 2014. Neotropical Monogenoidea. 58. Three new species of Gyrodactylus (Gyrodactylidae) from Scleromystax spp. (Callichthyidae) and the proposal of COII gene as an additional fragment for barcoding gyrodactylids. Folia Parasitol 61: 213-222.

BYKHOVSKAYA-PAVLOVSKAYA I et al. 1962. Key to parasites of freshwater fish of the USSR Akad. Nauk. Moscow: SSSR. 727pp.

CHAPMAN LJ et al. 2000. Ecology of a diplozoon parasite on the gills of the African cyprinid Barbus neumayeri. African Journal of Ecology 38: 312-320.

CRIBB TH & BRAY RA. 2010. Gut wash, body soak, blender and heat-fixation: approaches to the effective collection, fixation and preservation of trematodes of fishes. Systematic parasitology 76: 1-7.

DA CUNHA FP et al. 2020. Non-lethal molecular diagnostic for acanthocephalosis in Colossoma macropomum. Aquaculture 519: 734860.

DE NOIA M et al. 2022. Towards an in situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla. Parasitology 149: 605-611.

DUVAL E et al. 2021. Urine DNA (uDNA) as a non‐lethal method for endoparasite biomonitoring: Development and validation. Environmental DNA 3: 1035-1045.

EK-HUCHIM JP et al. 2012. Non-lethal detection of DNA from Cichlidogyrus spp. (Monogenea, Ancyrocephalinae) in gill mucus of the Nile tilapia Oreochromis niloticus. Diseases of aquatic organisms 98: 155-162.

EL HAFIDI F et al. 1998. Microhabitat distribution and coexistence of Microcotylidae (Monogenea) on the gills of the striped mullet Mugil cephalus: chance or competition? Parasitology Research 84: 315-320.

FERREIRA IA et al. 2019. Detection of Betanodavirus in experimentally infected European seabass (Dicentrarchus labrax, Linnaeus 1758) using non‐lethal sampling methods. Journal of fish diseases 42: 1097-1105.

FOLMER O et al. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294-299.

GILMORE S et al. 2010. The placement of Gyrodactylus salmonis (Yin & Sproston) in the molecular phylogeny of studied members of the Gyrodactylus wageneri‐group parasitizing salmonids. Journal of fish diseases 33: 461-467.

GUSSEV A. 1985. Parasitic metazoans: class Monogenea. In: BAUER ON. (Ed.) Key to the parasites of freshwater fish fauna of the USSR. Leningrad: Nauka. 424p.

HANSEN H et al. 2003. Mitochondrial DNA variation of Gyrodactylus spp. (Monogenea, Gyrodactylidae) populations infecting Atlantic salmon, grayling, and rainbow trout in Norway and Sweden. International Journal for Parasitology 33: 1471-1478.

HANSEN H et al. 2007. DNA taxonomy and barcoding of monogenean parasites: lessons from Gyrodactylus. Trends in parasitology 23: 363-367.

HENDERSON C et al. 2016. Assessing the suitability of a non-lethal biopsy punch for sampling fish muscle tissue. Fish physiology and biochemistry 42: 1521-1526.

IANNACONE J & ALVARIÑO L. 2012. Microecology of the monogenean Mexicana sp. on the gills of Anisotremus scapularis (Tschudi, 1846) (Osteichthyes, Haemulidae) of the marine coast of Lima, Peru. Neotropical Helminthology 6: 277-285.

IZJUMOVA N. 1956. Materials on the biology of Dactylogyrus extensus. Nybelin, Parazitologiya 16: 229-243.

JIN X et al. 2024. Description of three new species of Gyrodactylus von Nordmann, 1832 (Monogenea: Gyrodactylidae) on bitterling fishes (Acheilognathinae) from China. Parasitology International, 101: 102893.

KADLEC D et al. 2003. The microhabitat distribution of two Dactylogyrus species parasitizing the gills of the barbel, Barbus barbus. Journal of Helminthology 77: 317-325.

KRKOŠEK M et al. 2005. Nonlethal assessment of juvenile pink and chum salmon for parasitic sea lice infections and fish health. Transactions of the American Fisheries Society 134: 711-716.

KUMAR R et al. 2017. Spatial distribution of ectoparasites on the gills of the mullet, Liza macrolepis: the effects of pollution. Journal of Parasitic Diseases 41: 40-47.

KUUSELA J et al. 2008. Description of three new European cryptic species of Gyrodactylus Nordmann, 1832 supported by nuclear and mitochondrial phylogenetic characterization. Acta Parasitologica 53: 120-126.

MALMBERG G. 1970. The excretory systems and the marginal hooks as a basis for the systematics of Gyrodactylus (Trematoda, Monogenea). Arkiv for Zoologi 23: 1-235.

MASHALY M I et al. 2019. Microhabitat Selection of Ectoparasitic Monogenean Populations of the Nile Catfish, Clarias gariepinus. Jordan Journal of Biological Sciences 12: 573-580.

MIRGHAED AT et al. 2018. Parasite fauna of Chub, Squalius turcicus De Filippi, 1865 (Teleostei: Cyprinidae) from some rivers of the Southern Caspian Sea basin in Iran. Iranian Journal of Ichthyology 5: 109-117.

NORRIS L et al. 2020. Detection of the parasitic nematode, Pseudocapillaria tomentosa, in zebrafish tissues and environmental DNA in research aquaria. Journal of fish diseases 43: 1087-1095.

NITTA M. 2023. New Records of Monogeneans, Gyrodactylus cyprini (Gyrodactylidae) and Dactylogyrus extensus (Dactylogyridae), Parasitic on Reared Common Carp Cyprinus carpio (Cypriniformes: Cyprinidae) in Mie Prefecture, Japan. Species Diversity 28(2): 273-284.

ONDRAČKOVÁ M et al. 2020. Morphometric and genetic evidence for cryptic diversity in Gyrodactylus (Monogenea) infecting non-native European populations of Ameiurus nebulosus and A. melas. Parasitology 147: 1700-1711.

PALADINI G et al. 2009. The first report of Gyrodactylus salaris Malmberg, 1957 (Platyhelminthes, Monogenea) on Italian cultured stocks of rainbow trout (Oncorhynchus mykiss Walbaum). Veterinary parasitology 165: 290-297.

PALADINI G et al. 2014. Reservoir hosts for Gyrodactylus salaris may play a more significant role in epidemics than previously thought. Parasites & vectors 7: 1-13.

PAOLETTI M et al. 2018. Species-specific Real Time-PCR primers/probe systems to identify fish parasites of the genera Anisakis, Pseudoterranova and Hysterothylacium (Nematoda: Ascaridoidea). Fisheries Research 202: 38-48.

PIE MR et al. 2006. Density-dependent topographical specialization in Gyrodactylus anisopharynx (Monogenoidea, Gyrodactylidae): Boosting transmission or evading competition? Journal of Parasitology 92: 459-463.

PINACHO-PINACHO CD et al. 2021. Species delimitation of Gyrodactylus (Monogenea: Gyrodactylidae) infecting the southernmost cyprinids (Actinopterygii: Cyprinidae) in the New World. Parasitology Research 120: 831-848.

PLAISANCE L et al. 2007. The complete mitochondrial DNA sequence of the monogenean Gyrodactylus thymalli (Platyhelminthes: Monogenea), a parasite of grayling (Thymallus thymallus). Molecular and Biochemical Parasitology 154: 190-194.

RUBIO-GODOY M. 2008. Microhabitat selection of Discocotyle sagittata (Monogenea: Polyopisthocotylea) in farmed rainbow trout. Folia Parasitologica 55: 270-276.

SANDOVAL-GÍO JJ et al. 2008. Humoral antibody response of the tilapia Oreochromis niloticus against Cichlidogyrus spp. (Monogenea). Journal of Parasitology 94: 404-409.

SIMKOVÁ A et al. 2000. Co-existence of nine gill ectoparasites (Dactylogyrus: Monogenea) parasitising the roach (Rutilus rutilus L.): history and present ecology. International Journal for Parasitology 30: 1077-1088.

TAVARES GC et al. 2016. Nonlethal sampling methods for diagnosis of Streptococcus agalactiae infection in Nile tilapia, Oreochromis niloticus (L.). Aquaculture 454: 237-242.

TORCHIN ME & MITCHELL CE. 2004. Parasites, pathogens, and invasions by plants and animals. Frontiers in Ecology and the Environment 2: 183-190.

WOOTTEN R. 1974. The spatial distribution of Dactylogyrus amphibothrium on the gills of ruffe Gymnocephalus cernua and its relation to the relative amounts of water passing over the parts of the gills. Journal of Helminthology 48: 167-174.

XU P et al. 2014. Genome sequence and genetic diversity of the common carp, Cyprinus carpio. Nature genetics 46: 1212-1219.

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Publicado

2024-10-04

Como Citar

SHALAL, Rana. Detecção não letal de mtDNA do monogenético Gyrodactylus mutabilitas que registrou pela primeira vez no muco branquial de Cyprinus carpio, Iraque. Revista de Ciências Agroveterinárias, Lages, v. 23, n. 3, p. 554–562, 2024. DOI: 10.5965/223811712332024554. Disponível em: https://revistas.udesc.br/index.php/agroveterinaria/article/view/24712. Acesso em: 14 out. 2024.

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Seção

Artigo de Revisão - Ciência de Animais e Produtos Derivados