First genotyping confirmation of Pichia kudriavzevii in subclinically mastitic cows in Iraq

Authors

DOI:

https://doi.org/10.5965/223811712332024417

Keywords:

mycotic mastitis, bovine fungal infection, Candida krusei, conventional PCR, sequencing analysis

Abstract

Fungal pathogens exist obviously in environment; therefore, animals may act as a source of infection to human. Pichia kudriavzevii is ubiquitous fungus of lastly great attention due to its potential use in biotechnology and processing of food, and controversial safety. This study aims to determining the prevalence rate of subclinical mastitis in lactating cows, and demonstration the presence of Pichia kudriavzevii in milk of positively mastitic cows using the molecular phylogeny. Totally, 400 adult lactating cows were subjected for collection an approximately 50 ml of fresh milk that tested initially with the California Mastitis Test (CMT); and then, positive samples have tested molecularly using conventional polymerase chain reaction (PCR). Some molecularly positive samples were analyzed phylogenetically for confirming of local isolates in the National Centre for Biotechnology Information (NCBI). Overall, 54.25% cases were positively reacted by CMT. According to score of positivity, 83.87%, 11.98% and 4.15% were showed weak, distinct, and strong positive infections, respectively. Targeting the ITS region, 28.11% of samples were reacted positively to P. kudriavzevii at 278 bp. Phylogenetic analysis of eight local P. kudriavzevii isolates showed the nucleotide alignment similarity and substitutions. Phylogenetic tree analysis revealed that the local P. kudriavzevii isolates were showed a genetic identity to the NCI-BLAST P. kudriavzevii Mexico isolates (KY646192.1) at total genetic changes ranged 0.0035-0.005%. In conclusion, this represents first molecular phylogenic study in Iraq implicates the presence of P. kudriavzevii in subclinical mastitic cows. Nationwide surveys are useful in monitoring udder health, studying the impact of structural changes, and estimating the factor(s) contribute in incidence of disease and the role of different fungi in it.

 

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References

AIT-KAKI A et al. 2019. Evaluation of the prevalence of subclinical mastitis in dairy cattle in the Soummam Valley (Bejaia, Algeria). Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine 76: 143-148.

ALCAZAR-FUOLI L & MELLADO E. 2014. Current status of antifungal resistance and its impact on clinical practice. British Journal of Haematology 166: 471-484.

AL-EODAWEE EM et al. 2023. Molecular identification of Eimeria spp. and Eimeria bovis in water buffaloes, Iraq. Journal of Global Innovative Agricultural Sciences 11: 363-369.

ALGAMMAL AM et al. 2020. Prevalence, antimicrobial resistance profiles, virulence and enterotoxins-determinant genes of MRSA isolated from subclinical bovine mastitis in Egypt. Pathogens 9: 1-11.‏

ALVARADO CW et al. 2019. Factors of prevalence of subclinical mastitis in dairy cows in the district of Florida, Amazonas Region, Peru.‏ Revista de Investigaciones Veterinarias del Perú 30: 923-931.

ANUEYIAGU KN et al. 2022. Prevalence of Methicillin-resistant Staphylococcus aureus in Bovine Subclinical Mastitis in Jos South Local Government Area of Plateau State, Nigeria. European Journal of Veterinary Medicine 2: 7-11.‏

BEGEROW D et al. 2010. Current state and perspectives of fungal DNA barcoding and rapid identification procedures. Applied Microbiology and Biotechnology 87: 99-108.‏

BRILLOWSKA-DABROWSKA A & SINIECKA A. 2012. Molecular detection of Candida krusei. International Research Journal of Microbiology 3: 275-277.

CARPOURON JE et al. 2022. Emerging animal-associated fungal diseases. Journal of Fungi 8: 1-12.‏

CHEN X et al. 2022. Prevalence of subclinical mastitis among dairy cattle and associated risks factors in China during 2012–2021: A systematic review and meta-analysis. Research in Veterinary Science 148: 65-73.‏

CHU Y et al. 2023. Advances in the application of the non-conventional yeast Pichia kudriavzevii in food and biotechnology industries. Journal of Fungi 9: 1-21.

COBIRKA M et al. 2020. Epidemiology and classification of mastitis. Animals 10: 1-17.

CONSTABLE PD et al. 2016. Veterinary medicine-e-book: a textbook of the diseases of cattle, horses, sheep, pigs and goats. Elsevier Health Sciences. Pp: 1904-1964.

COSTA EO et al. 1998. Infectious bovine mastitis caused by environmental organisms. Journal of Veterinary Medicine Series B 45: 65–71.

DA SILVA BGM et al. 2022. Diphenyl diselenide suppresses key virulence factors of Candida krusei, a neglected fungal pathogen. Biofouling 38: 427-440.

DIESER AS et al. 2014. Prevalence of pathogens causing subclinical mastitis in argentinean dairy herds.‏ Pakistan Veterinary Journal 34: 124-126.

DOMÁN M et al. 2022. Molecular phylogenetic analysis of Candida krusei. Mycopathologia 187: 333-343.‏

DU J et al. 2018. Epidemiological investigation of non-albicans Candida species recovered from mycotic mastitis of cows in Yinchuan, Ningxia of China. BMC Veterinary Research 14: 1-9.‏

DWORECKA-KASZAK B et al. 2012. High prevalence of Candida yeast in milk samples from cows suffering from mastitis in poland. The Scientific World Journal 2012: 1-5.‏

EGGIMANN P et al. 2003a. Epidemiology of Candida species infections in critically ill non-immunosuppressed patients. The Lancet Infectious Diseases 3: 685-702.‏

EGGIMANN P et al. 2003b. Management of candidiasis Management of Candida species infections in critically ill patients. The Lancet Infectious Diseases 3: 772-785.‏

FESSEHA H et al. 2021. Study on prevalence of bovine mastitis and associated risk factors in dairy farms of Modjo town and suburbs, central Oromia, Ethiopia. Veterinary Medicine: Research and Reports 271-283.‏

FORASTIERO A et al. 2015. Rapid development of Candida krusei echinocandin resistance during caspofungin therapy. Antimicrobial Agents and Chemotherapy 59: 6975-6982.

GAUDIE C et al. 2009. Outbreak of disease due to Candida krusei in a small dairy herd in the UK. The Veterinary Record 165: 1-3.‏

GÓMEZ-QUISPE OE et al. 2015. Interpretation criteria for California Mastitis Test in the diagnosis of subclinical mastitis in cattle. Revista de Investigaciones Veterinarias del Perú 26: 86-95.

HAIDER A et al. 2023. Bovine Mastitis. In Polymeric Nanoparticles for Bovine Mastitis Treatment. Cham: Springer Nature Switzerland. Pp: 49-80.

HERNÁNDEZ-FERNÁNDEZ M et al. 2021. Culturable yeasts as biofertilizers and biopesticides for a sustainable agriculture: A comprehensive review. Plants 10: 1-19.

HIITIÖ H et al. 2017. Prevalence of subclinical mastitis in Finnish dairy cows: changes during recent decades and impact of cow and herd factors. Acta Veterinaria Scandinavica 59: 1-14.‏

HUSSEIN SA. 2012. Prevalence and bacterial etiology of subclinical mastitis in dairy cows in Al Sulaimaniyah district. Kufa Journal For Veterinary Medical Sciences 3: 190-203.

IWEN PC et al. 2002. Utilization of the internal transcribed spacer regions as molecular targets to detect and identify human fungal pathogens. Medical Mycology 40: 87-109.

JAMEEL FAR & YASSEIN SN. 2021. Virulence potential of Penicillium chrysogenum isolated from subclinical bovine mastitis. Iraqi Journal of Science 62: 2131-2142.‏

JAMIU AT et al. 2021. Update on Candida krusei, a potential multidrug-resistant pathogen. Medical Mycology 59: 14-30.

JASM MOHAMMED S & YASSEIN SN. 2020. Characterization of some virulence factors of Candida albicans isolated from subclinical bovine mastitis. Plant Archive 20: 238-242.‏

KAHIR MA et al. 2008. Prevalence and risk factors of subclinical bovine mastitis in some dairy farms of Sylhet district of Bangladesh. Korean Journal of Veterinary Service 31: 497-504.‏

KALAIARASAN K et al. 2018. Changing virulence factors among vaginal non-albicans Candida species. Indian Journal of Medical Microbiology 36: 364-368.

KHASANAH H et al. 2021. Subclinical mastitis: Prevalence and risk factors in dairy cows in East Java, Indonesia. Veterinary World 14: 2102–2108.‏

KIBEBEW K. 2017. Bovine mastitis: A review of causes and epidemiological point of view. Journal of Biology, Agriculture and Healthcare 7: 1-14.

KOÇYİĞİT R et al. 2016. Effect of some risk factors on subclinical mastitis in dairy cows. Kocatepe Veterinary Journal 9: 185-193.‏

KRUKOWSKI H. 2001. Mycotic mastitis in cows. Medycyna Weterynaryjna 57: 18-20.

KULIK T et al. 2020. Promising perspectives for detection, identification, and quantification of plant pathogenic fungi and oomycetes through targeting mitochondrial DNA. International Journal of Molecular Sciences 21: 1-22.‏

KUMAR P et al. 2020. Bovine mastitis: a review. Middle-East Journal of Scientific Research 28: 497-507.

MAALIK A et al. 2019. Prevalence and antibiotic resistance of Staphylococcus aureus and risk factors for bovine subclinical mastitis in District Kasur, Punjab, Pakistan. Pakistan Journal of Zoology 51: 1123-1130.‏

MBINDYO CM et al. 2020. Prevalence, etiology, and risk factors of mastitis in dairy cattle in Embu and Kajiado Counties, Kenya. Veterinary Medicine International 2020: 1-12.‏

MERSEGUEL KB et al. 2015. Genetic diversity of medically important and emerging Candida species causing invasive infection. BMC Infectious Diseases 15: 1-11.

MINNAT TR & HAMMADI KM. 2015. Detection of clinical and subclinical mastitis in dairy cows of Diyala Province, Iraq. Journal of Wasit for Science and Medicine 8: 71-81.

MOREIRA MA et al. 2019. Infectious diseases in dairy cattle. In Raw Milk. Academic Press. Pp: 235-258.

PAL M. 2023. Etiology, Transmission, Epidemiology, Clinical Spectrum, Diagnosis and Management of Fungal Mastitis in Dairy Animals: A Mini Review.‏ International Journal of Food Science and Agriculture 7: 424-429

PÉREZ-MORALES R et al. 2022. Factors associated with the prevalence of subclinical mastitis in double-purpose cattle. Abanico Veterinario 12: 1-16.‏

RADHY AM & SALMAN AH. 2015. Isolation of some fungal agents for subacute mastitis cows in AL-Anbar province. Iraqi Journal of Science 56: 345-349.‏

RAJA HA et al. 2017. Fungal identification using molecular tools: a primer for the natural products research community. Journal of Natural Products 80: 756-770.‏

REDDY BSS et al. 2014. Comparison of different diagnostic tests in subclinical mastitis in dairy cattle. International Journal of Veterinary Science 3: 224-228.

RIEKERINK RO et al. 2008. Incidence rate of clinical mastitis on Canadian dairy farms. Journal of Dairy Science 91: 1366-1377.‏

RIFATBEGOVIĆ M et al. 2024. Pathogens Associated with Bovine Mastitis: The Experience of Bosnia and Herzegovina. Veterinary Sciences 11: 1-12.

SADOON AS et al. 2011. Isolation and identification of some bacteria causing subclinical mastitis in cows. Iraqi Journal of Veterinary Sciences 25: 63-67.

SAEED SI et al. 2022. Prevalence, antimicrobial resistance, and characterization of Staphylococcus aureus isolated from subclinical bovine mastitis in East Coast Malaysia. Animals 12: 1-11.‏

SALEEM HD et al. 2021. Cumulative Effect of Subclinical Mastitis on Immunological and Biochemical Parameters in Cow Milk. Archives of Razi Institute 76: 1599-1608.

SARTORI LCA et al. 2014. Identification of Candida species isolated from cows suffering mastitis in four Brazilian states. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 66: 1615-1617.‏

SHARMA N et al. 2013. Status of bovine mastitis and associated risk factors in subtropical Jeju Island, South Korea. Tropical Animal Health and Production 45: 1829-1832.‏

SHRUTHI B et al. 2022. Exploring biotechnological and functional characteristics of probiotic yeasts: A review. Biotechnology Reports 34: e00716.

SONMEZ M & ERBAS G. 2017. Isolation and identification of Candida spp. from mastitis cattle milk and determination of antifungal susceptibilities.‏ International Journal of Veterinary Science 6: 104-107

THEMISTOKLEOUS K et al. 2019. Epidemiological evaluation of subclinical mastitis of dairy cows in Greece. Journal of the Hellenic Veterinary Medical Society 70: 1865-1874.‏

UMAM AAK et al. 2017. Study on the bulk milk somatic cell counts and milk quality in different seasons. Scholar Journal of Agricultural and Veterinary Sciences 4: 498-503.

WU Y et al. 2020. Antifungal activity and mode of action of miltefosine against clinical isolates of Candida krusei. Frontiers in Microbiology 11: 537742.

YADAV JSS et al. 2012. Candida krusei: biotechnological potentials and concerns about its safety. Canadian Journal of Microbiology 58: 937-952.

ZARAGOZA CS et al. 2011. Yeasts isolation from bovine mammary glands under different mastitis status in the Mexican High Plateu. Revista Iberoamericana De Micologia 28: 79-82.‏

ZHANG LJ et al. 2019. Polymorphism analysis of virulence-related genes among Candida tropicalis isolates. Chinese Medical Journal 132: 446-453.

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Published

2024-10-04

How to Cite

GHARBAN, Hasanain A. J. First genotyping confirmation of Pichia kudriavzevii in subclinically mastitic cows in Iraq. Revista de Ciências Agroveterinárias, Lages, v. 23, n. 3, p. 417–424, 2024. DOI: 10.5965/223811712332024417. Disponível em: https://revistas.udesc.br/index.php/agroveterinaria/article/view/25396. Acesso em: 12 dec. 2024.

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Section

Research Article - Science of Animals and Derived Products