Genotypic profile and antimicrobial resistance of avian pathogenic Escherichia coli

Authors

DOI:

https://doi.org/10.5965/223811712312024070

Keywords:

epidemiology, susceptibility, poultry, bacterium

Abstract

Avian pathogenic Escherichia coli (APEC) is responsible for several anatomopathological conditions in poultry, which cause great damage to the poultry sector. This study aimed to evaluate the pathogenicity of E. coli strains isolated from broiler chickens and to perform in vitro tests on strains classified as APEC to identify their capacity to form biofilms and sensitivity to antimicrobials routinely used in the poultry breeding process. Sixty E. coli poultry isolated were used in this study. The real-time Polymerase Chain Reaction (PCR) test identified that 100% of the isolates had the hlyF and ompT genes, 98.3% the iroN and iss genes, and 83.33% the iutA gene, being all the evaluated strains classified as APEC. In the in vitro evaluations regarding the formation and characterization of biofilms, the APEC samples were characterized as 71.66% weakly biofilm-forming. The antibiotic sensitivity test showed that the highest resistance percentages were found in the molecules of oxytetracycline, with 33%, and doxycycline, with 31.66%, but about 58,33% of the samples had a profile of multi-resistance to antimicrobials. Further studies are needed to better characterize APEC virulence genes and multi-drug resistance, given their impact on poultry health/production and potential risk to human health.

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Author Biographies

Ana Carolina Spricigo, Instituto Federal Catarinense, Araquari e Concórdia, SC, Brasil.

Mestre em Produção e Sanidade Animal pelo Instituto Federal Catarinense, Campus Concórdia

Vanessa Peripolli, Instituto Federal Catarinense, Araquari e Concórdia, SC, Brasil.

Zootecnista

Paulo Mafra de Almeida Costa, Instituto Federal Catarinense, Araquari e Concórdia, SC, Brasil.

Engenheiro Agrônomo

Alessandra Millezi, Instituto Federal Catarinense, Araquari e Concórdia, SC, Brasil.

Licenciada em Ciências Biológicas pela Universidade Regional e Integrada do Alto Uruguai e das Missões, Especialista em Educação pela Universidade Federal de Lavras, Mestre e Doutora em Microbiologia Agrícola pela Universidade Federal de Lavras. Docente do Ensino B´´asico Técnico e Tecnológico do Instituto Federal de Ciência e Tecnologia Catarinense, Campus Concórdia.

Jhonatan Sperandio, Instituto Federal Catarinense, Araquari e Concórdia, SC, Brasil.

Zootecnista

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Published

2024-04-01

How to Cite

SPRICIGO, Ana Carolina; PERIPOLLI, Vanessa; COSTA, Paulo Mafra de Almeida; MILLEZI, Alessandra; SPERANDIO, Jhonatan. Genotypic profile and antimicrobial resistance of avian pathogenic Escherichia coli. Revista de Ciências Agroveterinárias, Lages, v. 23, n. 1, p. 70–78, 2024. DOI: 10.5965/223811712312024070. Disponível em: https://revistas.udesc.br/index.php/agroveterinaria/article/view/24792. Acesso em: 22 jul. 2024.

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Section

Research Article - Science of Animals and Derived Products

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