Efeito da bioatividade de extratos de Elephantopus Scaber Linn. em Spodoptera litura e na comunidade microbiana do solo





Bioatividade, Biopesticida, Estrutura da comunidade, Mortalidade, Nível de proteína


Este estudo tem como objetivo investigar os efeitos biopesticidas de Elephantopus scaber Linn. extrair na mortalidade de Spodoptera litura, Plutella xylostella e organismos não-alvo e investigar o impacto nos níveis de proteína de S. litura e na estrutura da comunidade microbiana do solo. Experimento realizado em delineamento inteiramente casualizado. Extratos de metanol de folhas de E. scaber, nas concentrações de 2%, 4%, 6%, 8%, 10% e 12%, foram testados para bioatividade contra a larva de 2º ínstar de S. litura, P. xylostella e minhocas. Taxas de mortalidade de larvas e vermes foram observadas. Os dados coletados foram analisados ​​por meio de análise de variância (ANOVA), seguida de probit e análise descritiva. Os resultados mostraram que os extratos metanólicos de E. scaber (12%) influenciaram as maiores taxas de mortalidade para S. litura (93,35%) e P. xylostella (96,65%) com CL50 e CL80 de S. litura foi de 1,867 e 4,763; para P. xylostella foram 4,488 e 7,92, respectivamente. No entanto, a aplicação de biopesticida de E. scaber também influencia a taxa de mortalidade de minhocas. O extrato de 6% de E. scaber resultou em 60% da morte de minhocas durante o período de 20 dias. Para além disso, maiores concentrações de extratos de E. scaber resultaram em menores pesos moleculares e níveis de proteínas de S. litura. A diversidade e a densidade da comunidade microbiana do solo também diminuíram na concentração de 6%.


Não há dados estatísticos.


ABDUL RW et al. 2018. Plant defense against herbivory and insect adaptations. AoB PLANTS 10: 1-19.

BALFAS R & WILLIS M. 2009. Pengaruh ekstrak tumbuhan obat terhadap mortalitas dan kelangsungan hidup Spodoptera litura F. (Lepidoptera, Noctuidae). Bulletin LITTRO 20: 148-156.

BASKAR KR et al. 2011. Bioefficacy of Couroupita guianensis (Aubl) against Helicoverpa armigera (Hub. (Lepidoptera: Noctuidae) Larvae. Spanish Journal of Agricultural Research 8: 135-141.

BIDLACK WR. 2000. Phytochemicals as bioactive agents. Lancaster: Tehnomic Publishing Company, Inc.

BRISCA R & KITHERIAN S. 2009. Influence of botanical in total head protein of Spodoptera litura (Fab.). Journal of Biopesticides 2: 52-55.

CHANDLER D et al. 2011. The development, regulation and use of biopesticides for integrated pest management. Philosophical Transactions of the Royal Society B 366: 1987-1998.

COTTRELL HJ. 1987. Pesticides on plant surfaces. New York: Society of Chemichal Industry.

CRUZ PHG et al. 2018. Insecticidal property and phytochemical screening of mahogany (Swietenia macrophylla King) leaves, barks and seeds as an alternative insecticide against fungi growing termites. International Journal of Biology Pharmacy and Allied Sciences 7: 1521-1528.

DAISY P et al. 2008. A novel terpenoid from Elephantopus scaber - antibacterial activity on Staphylococcus aureus: A substantiate computational approach. International Journal of Biology Pharmacy and Allied Sciences 4: 196-203.

DAMIEN D & RAIMO VKH. 2004. Fe (III) reductive and free radical-scavenging properties of summer savory (Satureja hortensis L.) extract and subfractions. Food Chemistry 88: 1887-1892.

DAWKAR VV. 2019. Azadirachtin-A from Azadirachta indica Impacts Multiple Biological Targets in Cotton Bollworm Helicoverpa armigera. ACS Omega 4: 9531-9541.

FARHA AK & REMANI P. 2014. Phytopharmacological profile of Elephantopus scaber. Pharmacologia 5: 272-285.

FATCHIYAH F et al. 2011. Biologi molekular prinsip dasar analisis. Jakarta: Erlangga.

FREDERICK MA et al. 2003. Current protocols in molecular biology. New York: John Wiley & Sons.

GOKULAKRISHNAN J et al. 2012. Certain plant essential oils againts antifeedant activity of Spodoptera litura (Fab.), Helicoverpa armigera (Hub.) and Achaea janata (Linn.) (Lepidoptera: Noctuidae). International Journal of Current Life Sciences 2: 5-11.

HUANG Z et al. 2004. Protein metabolism in Spodoptera litura (F.) is influenced by the botanical insecticide azadirachtin. Pesticide Biochemistry and Physiology 80: 85-93.

HUANG Z et al. 2007. Effects of Azadirachtin on Hemolymph Protein Expression in Ostrinia furnacalis (Lepidoptera: Crambidae). Annals of the Entomological Society of America 100: 245-250.

HUSSAIN S & SALEEM M. 2009. Impact of peticides on soil microbial diversity, enzyme and biochemical reaction. Advances in Agronomy 102: 159-200.

JURG BL et al. 2015. Editorial: Microbial response to environmental changes. Front microbiol 6: 1-4.

KATHIRVELU B et al. 2010. Bioefficacy of Couroupita guianensis (Aubl) against Helicoverpa armigera (Hub. (Lepidoptera: Noctuidae) larvae. Spanish Journal of Agricultural Research 8: 135-141.

KORTBEEK RWJ et al. 2019. Endogenous plant metabolites against insects. European Journal of Plant Pathology 154: 67-90.

KUMAR S et al. 2004. Antibacterial studies on leaf extract of Elephantopus scaber Linn. Ancient science of life 23: 6-8.

KUMAR S et al. 2008. Antioxidant and free radical scavenging potential of Citrullus colocynthis (I.) Schrad. methanolic fruit extract. Acta Pharmaceutica 58: 215-220.

KUMAR S. 2012. Biopesticides: A need for food and environmental safety. Journal of Biofertilizers and Biopesticides 3: 1000e107.

KUSNADI O. 2003. Pengendalian hama tanpa pestisida. Jakarta: Penebar Swadaya.

LARRY PP. 2002. Entomology and pest management. Upper Saddle River: Prentice Hall.

LI W et al. 2004. Chemical composition of the essential oil of E. scaber from Southern China. Zeitschrift für Naturforschung C J Biosci 59: 327-329.

LU FC & KACEW S. 2002. Lu’s basic toxicology: Fundamentals, target organs and risk management. 4.ed. London: Taylor and Francis.

MADIGAN MT et al. 2003. Biology of microorganisms. Englewood Cliffs, New Jersey: Prentice Hall.

MOHAN VR et al. 2010. Pharmacognostic and phytochemical investigation of E. scaber L. (Asteraceae). Journal of Pharmaceutical Science and Technology 2: 191-197.

MOSSA ATH. 2016. Green pesticides: Essential oils as biopesticides in insect-pest management. Journal of Environmental Science and Technology 9: 354-378.

OLESZCZUK P et al. 2014. Effect of pesticides on microorganism enzymatic activity and plant in biochar-amended soil. Geoderma 214-215: 10-18.

RACHMADIARTI F et al. 2019. Total Phenolic and Flavonoid Contents of Elephantopus scaber and Ageratum conyzoides (Asteraceae) Leaves Extracts on Various Altitude Habitats. Ecology, Environment and Conservation 25: 84-91.

REGNAULT-ROGER C. 1997. The potential of botanical essential oils for insect pest control. Integrated Pest Management Reviews 2: 25-34.

ROBINSON T. 1975. The organic constituents of higher plants: Their chemistry and interrelationship. 3.ed. North Amherst, Massachusetts: Cordus Press.

SENGOTTAYAN SN. 2015. A Review of Biopesticides and Their Mode of Action Against Insect Pests. In: THANGAVEL P & SRIDEVI G. (eds). Environmental Sustainability. India: Springer.

SETHI S & SAKSHAM G. 2013. Impact of pesticides and biopesticides on soil microbial biomass carbon. Universal Journal of Environmental Research & Technology 3: 326-330.

SHAHABI S et al. 2007. Environmental Factors can Modify Genotype Risks by Slight Changes in Protein Conformation: The Role of Water. Environmental Factors.

SHAHRAM S et al. 2008. Environmental factors can modify genotype risks by slight changes in protein conformation: The role of water. [online] Availale at: ttps://pdfs.semanticscholar.org/1320/967fe72b7c7bcec1a2c80e7936143223f672.

pdf?_ga=2.68659537.1780640978.1597311159-1921116365.1536741116. Accessed: August 2020.

SINGLETON VL et al. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin ciocalteu reagent. Methods in Enzymology 299: 152-178.

STANOJEVIĆ L et al. 2009. Antioxidant activity and total phenolic and flavonoid contens of Hieracium pilosella L. Sensors (Basel) 9: 5702-5714.

TEHRI K & SINGH N. 2015. The Role of Botanicals as Green Pesticides in integrated mosquito management - A review. International Journal of Mosquito Research 2: 18-23.

TRAITHIP A. 2005. Phytochemistry and antioxidant Activity of P. Indica. Salaya. Thailand: Mahidol University.

WIJAYA S et al. 2011. Antioxidant, anti-inflammatory, cytotoxicity and cytoprotection activities of Crassocephalum crepidioides (Benth.) S. Moore. extracts and its phytochemical composition. European Journal of Scientific Research 67: 157-165.

WINK M. 2010. Biochemistry of plant secondary metabolism: Annual plant review 40. Heidelberg: Wiley Blackwell.

YULIANI & RAHAYU YS. 2017. Journal of Physics: Conference Series. The using of fenolic compounds of Pluchea indica less leaves extracts as a bioinsecticide and bioherbicide: Proceedings of the 2nd International Joint Conference on Science and Technology (IJCST). Bali, Indonesia: IOP Publishing.

YULIANI et al. 2018. Atlantis Highlights in Engineering. The morphological, anatomical, and physiological characteristics of Elephantopus scaber as explant source for tissue culture: Proceeding of the International Conference on Science and Technology (ICST 2018). Yogyakarta, Indonesia: Atlantis Press.

YULIANI et al. 2019. Total Phenolic and Flavonoid contents of Elephantopus scaber and Ageratum conyzoides (Asteraceae) Leaves extracts from Various Altitude Habitats. Journal Ecology, Environment and Conservation 25: 106-113.




Como Citar

YULIANI, Yuliani. Efeito da bioatividade de extratos de Elephantopus Scaber Linn. em Spodoptera litura e na comunidade microbiana do solo. Revista de Ciências Agroveterinárias, Lages, v. 21, n. 3, p. 206–215, 2022. DOI: 10.5965/223811712132022206. Disponível em: https://revistas.udesc.br/index.php/agroveterinaria/article/view/21500. Acesso em: 18 maio. 2024.



Artigo de Pesquisa - Ciência de Plantas e Produtos Derivados