Semen characterization of tambaqui (Colossoma macropomum) during the breeding season

Authors

  • Dayane Regina Lenz Universidade Federal da Fronteira Sul
  • André de Moura Victorio Universidade Federal da Fronteira Sul
  • Mayanny Carla Carvalho Lima Universidade Federal de Goiás
  • Tayrone Freitas Prado Universidade Federal de Goiás
  • Fernanda Gomes de Paula Universidade Federal de Goiás
  • Maria Lucia Gambarini Meirinhos Universidade Federal de Goiás
  • Emmanuel Arnhold Universidade Federal de Goiás

DOI:

https://doi.org/10.5965/223811711732018603

Keywords:

Characidae, espermatozoa, semen analysis

Abstract

This study aims to qualitatively and quantitatively characterize the semen of tambaqui (Colossoma macropomum) during the breeding season. Three samplings were made at regular intervals of approximately 30 days in the 2012/2013 breeding season. Ten adult males of at least three years old age were selected. To collect the semen, hormonal induction was performed with carp pituitary extract, and extraction occurred by abdominal massage. After collection, the semen was subjected to evaluation of motility, vigor, lifetime, ejaculate volume, and sperm concentration. Analysis of variance (ANOVA) and Tukey test were applied on quantitative variables, while the Freedman test was used with 0.05 significance on qualitative variables. No significant differences (p>0.05) were observed for most parameters evaluated. Sperm concentration significantly varied between samples (14.5, 3.7, and 8.3x109 sperm/mL) and lifetime decreased significantly between the second and third samples (267.8 e 98.5 seconds, respectively).

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Author Biographies

Dayane Regina Lenz, Universidade Federal da Fronteira Sul

Engenheiro de pesca pela UNIOESTE.

Mestre em Ciência Animal pela Universidade Federal de Goiás.

André de Moura Victorio, Universidade Federal da Fronteira Sul

Médico Veterinário pela Universidade Federal de Santa Maria,

Mestrando em Agroecologia e Desenvolvimento Rural pela Universidade Federal da Fronteira Sul

Mayanny Carla Carvalho Lima, Universidade Federal de Goiás

Zootecnista.

Mestre em Ciência Animal pela UFG.

Fernanda Gomes de Paula, Universidade Federal de Goiás

Prof. Dr. da Escola de Veterinária e Zootecnia

Maria Lucia Gambarini Meirinhos, Universidade Federal de Goiás

Prof. Dr. da Escola de Veterinária e Zootecnia

Emmanuel Arnhold, Universidade Federal de Goiás

Prof. Dr. da Escola de Veterinária e Zootecnia

References

BILLARD R & COSSON MP. 1992. Some problems related to the assessment of sperm motility in freshwater fish. Journal of Experimental Zoology 261: 122-131.

BILLARD R et al. 1995. Biology of sperm and artificial reproduction in carp. Aquaculture 129: 95-112.

GALLEGO V et al. 2017. Fish sperm sub populations: Changes after cryo preservation process and relationship with fertilization success in tambaqui (Colossoma macropomum). Theriogenology 87: 16-24.

GOES MD et al. 2017. Natural and artificial spawning strategies with fresh and cryopreserved semen in Rhamdia quelen: Reproductive parameters and genetic variability of offspring. Theriogenology 88: 254-263.

GOMES LC et al. 2010. Tambaqui (Colossoma macropomum). In: BALDISSEROTO B & GOMES LV (Org.). Espécies nativas para piscicultura no Brasil. 2.ed. Santa Maria: UFSM. p.175-204.

LAHNSTEINER F. 2003. Morphology, fine structure, biochemistry, and function of the spermatic ducts in marine fish. Tissue Cell 35: 363-373.

LEITE LV et al. 2013. Determinação da dose inseminante e embriogênese na fertilização artificial de tambaqui (Colossoma macropomum). Arquivo Brasileiro de Medicina Veterinária e Zootecnia 65: 421-429.

MARIA AN et al. 2010. Semen characterization and sperm structure of the Amazon tambaqui (Colossoma macropomum). Journal of Applied Ichthyology 26: 779-783.

MARTINS EFF et al. 2017. Ovopel and carp pituitary extract for the reproductive induction of Colossoma macropomum males. Theriogenology 98: 57-61.

MYLONAS CC et al. 2010. Broodstock management and hormonal manipulations of fish reproduction. General and Comparative Endocrinology 165: 516-534.

MYLONAS CC et al. 2017. Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality. Aquaculture 472: 21-44.

PIRES LB et al. 2017. Semen characteristics of Colossoma macropomum from three successive sample colections in the same reproductive cycle. Aquaculture Research 48: 5104-5110.

R DEVELOPMENT CORE TEAM. 2012. R: A language and environment for statistical computing [online]. R Foundation for Statistical Computing. Disponível em: http://www.R-project.org/. Acesso em: 30 mar. 2012.

ROMAGOSA E et al. 2010. Sperm motility of Prochilodus lineatus in relation to dilution rate and temperature of the activating medium. Journal of Applied Ichthyology 26: 678-681.

SALMITO-VANDERLEY CSB et al. 2015. Conservação de gametas e embriões de peixes teleósteos. Revista Brasileira de Reprodução Animal 39: 184-188.

SANCHES EA et al. 2009. Dose inseminante para fertilização artificial de ovócitos de dourado. Revista Brasileira de Zootecnia 38: 2091-2098.

SANCHES EA et al. 2010. Sperm motility of Rhamdia quelen studied using computer assisted analysis by open-source software. Aquaculture Research 42: 153-156.

SHULZ RW et al. 2010. Spermatogenesis in fish. General and Comparative Endocrinology 165: 390-411.

STREIT JÚNIOR DP et al. 2006. Características qualitativas do sêmen de pacu (Piaractus mesopotamicus) após indução hormonal. Bioscience Journal 22: 119-125.

VIEIRA MJAF et al. 2011. Características do sêmen de tambaqui (Colossoma macropomum) em latitude equatorial. Archivos de Zootecnia 60: 1263-1270.

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Published

2018-11-14

How to Cite

LENZ, Dayane Regina; VICTORIO, André de Moura; LIMA, Mayanny Carla Carvalho; PRADO, Tayrone Freitas; DE PAULA, Fernanda Gomes; MEIRINHOS, Maria Lucia Gambarini; ARNHOLD, Emmanuel. Semen characterization of tambaqui (Colossoma macropomum) during the breeding season. Revista de Ciências Agroveterinárias, Lages, v. 17, n. 4, p. 603–607, 2018. DOI: 10.5965/223811711732018603. Disponível em: https://revistas.udesc.br/index.php/agroveterinaria/article/view/10131. Acesso em: 21 dec. 2024.

Issue

Vol. 17 No. 4 (2018)

Section

Research Note - Science of Animals and Derived Products

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Copyright (c) 2018 Revista de Ciências Agroveterinárias (Journal of Agroveterinary Sciences)

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