Menthol as a natural anesthetic for guppy
DOI:
https://doi.org/10.5965/223811711922020249Keywords:
anesthetic, induction, ornamental fish, Poecilia reticulata, recoveryAbstract
The objective of this study was to evaluate different menthol concentrations for the anesthesia of adult females, adult males, and juvenile guppy (Poecilia reticulata). To this end, 30 adult females (0.379 ± 0.108 g), 30 adult males (0.220 ± 0.049 g), and 30 juveniles (0.033 ± 0.016 g) were used. The animals were individually exposed to five concentrations of menthol (50, 100, 150, 200, and 250 mg L-1), six fish per concentration. The induction times, anesthetic recovery, and mortality up to 96 h after the experiment were evaluated. The concentration of 50 mg L-1 did not induce anesthesia in any of the studied groups. The concentrations of 100 and 250 mg L-1 resulted in 100% mortality in the adults (male and female) and juveniles, respectively. The concentrations from 150 to 250 mg L-1 and from 150 to 200 mg L-1 of menthol were efficient and safe for use as anesthesia for guppy adults (male and female) and juveniles, respectively.
Downloads
References
BALAMURUGAN J et al. 2016. Clove extract: A potential source for stress free transport of fish. Aquaculture 454: 171-175.
BOLASINA SN et al. 2017. Comparative efficacy of benzocaine, tricaine methanesulfonate and eugenol as anesthetic agents in the guppy Poecilia Vivipara. Aquaculture Reports 6: 56-60.
BOYD CE & TUCKER CS. 1998. Pond aquaculture water quality management. 1.ed. New York: Springer Science & Business Media.
CHAMBEL J et al. 2015. The efficacy of MS‐222 as anaesthetic agent in four freshwater aquarium fish species. Aquaculture Research 46: 1582-1589.
CUNHA L et al. 2015. Clove oil as anesthetic for guppy. Boletim do Instituto de Pesca 41: 729-735.
DOLEŽELOVÁ P et al. 2011. The acute toxicity of clove oil to fish Danio rerio and Poecilia reticulata. Acta Veterinaria Brno 80: 305-308.
FAÇANHA MF & GOMES LC. 2005. Efficacy of menthol as an anesthetic for tambaqui (Colossoma macropomum, Characiformes: Characidae). Acta Amazonica 35:71-75.
GONÇALVES AFN et al. 2008. Mentol e eugenol como substitutos da benzocaína na indução anestésica de juvenis de pacu. Acta Scientiarum Animal Sciences 30: 339-344.
HOSHIBA MA et al. 2015. Clove oil and menthol as anesthetic for platy. Boletim do Instituto de Pesca 41: 737-742.
KEENE JL et al. 1998. The efficacy of clove oil as an anaesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture Research 29: 89-101.
OKAMURA D et al. 2010. Influência da concentração de benzocaína e do comprimento dos peixes na anestesia e na recuperação de tilápias-do-nilo. Revista Brasileira de Zootecnia 39: 971-976.
OSTRENSKY A et al. 2016. Use of Clove, Mint and Camphor Essential Oils on Confinement of Clown Anemonefish Amphiprion ocellaris (Cuvier 1830): Anesthetic Effects and Influence on Water Quality. Journal of Aquaculture Research & Development 7:457.
PÁDUA SB et al. 2010. Mentol como anestésico para dourado (Salminus brasiliensis). Boletim do Instituto de Pesca 36: 143-148.
PARODI TV et al. 2014. Anesthetic activity of the essential oil of Aloysia triphylla and effectiveness in reducing stress during transport of albino and gray strains of silver catfish, Rhamdia quelen. Fish Physiology and Biochemistry 40: 323-334.
PATTANASIRI T et al. 2016. Acute toxicity and anaesthetic effect of clove oil and eugenol on Siamese fighting fish, Betta splendens. Aquaculture International 25: 163-175.
PEDRAZZANI AS & OSTRENSKY NETO A. 2016. The anaesthetic effect of camphor (Cinnamomum camphora), clove (Syzygium aromaticum) and mint (Mentha arvensis) essential oils on clown anemonefish, Amphiprion ocellaris (Cuvier 1830). Aquaculture research 47: 769-776.
PEREIRA‐DA‐SILVA EM et al. 2016. Menthol as anaesthetic for lambari Astyanax altiparanae (Garutti & Britski 2000): attenuation of stress responses. Aquaculture research 47: 1413-1420.
ROMANELI RS et al. 2018. Efficacy of Benzocaine, Eugenol, and Menthol as Anesthetics for Freshwater Angelfish. Journal of Aquatic Animal Health 30: 210-216.
ROSS LG & ROSS B. 2008. Anaesthetic and sedative techniques for aquatic animals. 3.ed. Oxford: Blackwell. 340p.
SIMÕES LN & GOMES LC. 2009. Eficácia do mentol como anestésico para juvenis de tilápia-do-nilo (Oreochromis niloticus). Arquivo Brasileiro de Medicina Veterinária e Zootecnia 61: 613-620.
SOUZA RAR et al. 2012. Efeito comparativo da benzocaína, mentol e eugenol como anestésicos para juvenis de robalo peva. Boletim do Instituto de Pesca 38: 247-255.
STEVENS CH et al. 2017. Stress and welfare in ornamental fishes: what can be learned from aquaculture? Journal of fish biology 9: 1409-428.
TEIXEIRA EG. et al. 2011. Mentol como anestésico para diferentes classes de tamanho de tilápia do nilo. Archives of Veterinary Science 16: 75-83.
WONG D. et al. 2014. Conditioned Place Avoidance of Zebrafish (Danio rerio) to Three Chemicals Used for Euthanasia and Anaesthesia. PLoS ONE 9: e88030.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Revista de Ciências Agroveterinárias (Journal of Agroveterinary Sciences)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors publishing in this journal are in agreement with the following terms:
a) Authors maintain the copyrights and concede to the journal the copyright for the first publication, according to Creative Commons Attribution Licence.
b) Authors have the authority to assume additional contracts with the content of the manuscript.
c) Authors may supply and distribute the manuscript published by this journal.
